한국초파리연구자원은행 KDRC > K-Gut Project

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Split Gal4 Images
K-GUT ID JK R65D06 X R57F07
DBD
BDSC ID 69430 FlyLight ID R65D06 Associated Gene AstA
AD
BDSC ID 71151 FlyLight ID R57F07 Associated Gene Dh31
Crop X PV X R1 X
R2 X R3 X R4 X
R5 X MHJ X Hindgut X
Rectum X

Split-Gal4 associated image

Associated Split Gal4 Images
K-GUT ID DBD AD
BDSC ID FlyLight ID Associated Gene BDSC ID FlyLight ID Associated Gene
JK R61H08 X R57F07 69158 R61H08 AstC 71151 R57F07 Dh31
JK R57F07 X R65D05 70283 R57F07 Dh31 69989 R65D05 AstA
JK R57F07 X R67F03 70283 R57F07 Dh31 70786 R67F03 AstC
JK R57F07 X R17D02 70283 R57F07 Dh31 69078 R17D02 fkh
JK R57F07 X R46D01 70283 R57F07 Dh31 70700 R46D01 hth
JK R57F07 X R27E07 70283 R57F07 Dh31 69855 R27E07 l(2)gl
JK R57F07 X R60H02 70283 R57F07 Dh31 68901 R60H02 MsR1
JK R57F07 X R16E08 70283 R57F07 Dh31 68946 R16E08 noc
JK R57F07 X R69H11 70283 R57F07 Dh31 71120 R69H11 vnd
JK R65D05 X R57F07 69667 R65D05 AstA 71151 R57F07 Dh31
JK R61H08 X R57F07 69158 R61H08 AstC 71151 R57F07 Dh31
JK R67F03 X R57F07 69456 R67F03 AstC 71151 R57F07 Dh31
JK R79E07 X R57F07 69697 R79E07 CG9650 71151 R57F07 Dh31
JK R57F07 X R57F07 70283 R57F07 Dh31 71151 R57F07 Dh31
JK R11F03 X R57F07 69178 R11F03 Fas2 71151 R57F07 Dh31
JK R17D02 X R57F07 69173 R17D02 fkh 71151 R57F07 Dh31
JK R26C12 X R57F07 69726 R26C12 Fur1 71151 R57F07 Dh31
JK R27E07 X R57F07 69810 R27E07 l(2)gl 71151 R57F07 Dh31
JK R60E02 X R57F07 69533 R60E02 NPFR 71151 R57F07 Dh31
JK R40F12 X R57F07 69935 R40F12 pros 71151 R57F07 Dh31
JK R20D06 X R57F07 69572 R20D06 sNPF 71151 R57F07 Dh31
JK R33A12 X R57F07 68537 R33A12 Src64B 71151 R57F07 Dh31
JK R34E04 X R57F07 69054 R34E04 tws 71151 R57F07 Dh31
JK R69H11 X R57F07 69819 R69H11 vnd 71151 R57F07 Dh31

Gal4 associated image

Associated Gal4 Images
K-GUT ID Gal4
BDSC ID FlyLight ID Associated Gene
GS39352_1 39352 R65D06 AstA

K-GUT Project

K-GUT project is an on-going project of KDRC, which aims to archive Drosophila gut GAL4 transgenes and their expression patterns. K-GUT project provides a database accessible to all researchers in the Drosophila community.

Principal Investigators Participating in K-GUT Project

Greg S. B. Suh
KAIST, seongbaesuh@kaist.ac.kr
Jae Young Kwon
Sungkyunkwan University, jykwon@skku.edu
Won-Jae Lee
Seoul National University, lwj@snu.ac.kr
Young-Joon Kim
GIST, kimyj@gist.ac.kr

Publication

Seung Yeon Lim, Hyejin You, Jinhyeong Lee, Jaejin Lee, Yoojin Lee, Kyung-Ah Lee, Boram Kim, Ji-Hoon Lee, JiHyeon Jeong, Sooin Jang, Byoungsoo Kim, Hyungjun Choi, Gayoung Hwang, Min Sung Choi, Sung-Eun Yoon, Jae Young Kwon, Won-Jae Lee, Young-Joon Kim & Greg S. B. Suh (2020) Identification and characterization of GAL4 drivers that mark distinct cell types and regions in the Drosophila adult gut, Journal of Neurogenetics, DOI: 10.1080/01677063.2020.1853722
For reprint requests, kdrc@gist.ac.kr

Imaging Conditions

K-GUT ID GS JK WL LS
Microscopy Zeiss LSM 780 Zeiss LSM 700 Zeiss LSM 700 Zeiss Lightsheet Z.1
Lens 20X
(Plan-Apochromat 20x/0.8 M27)
20X 20X
(Plan Apochromat 20x/0.8)
10X
(W Plan-Apochromat 10x/0.5)
Antibodies GFP (Invitrogen, A11122) GFP (Invitrogen, A11122) GFP (Invitrogen, A11122) GFP (Invitrogen, A6455)
Prospero (DSHB, MR1A) Prospero (DSHB, MR1A) Prospero (DSHB, MR1A)
AlexaFluor-488
(Invitrogen, A21206)
AlexaFluor-488
(Invitrogen, A11034)
AlexaFluor-488
(Invitrogen, A11008)
AlexaFluor-488
(Invitrogen, A32723)
AlexaFluor-594
(Invitrogen, A21203)
AlexaFluor-568
(Invitrogen, A11031)
AlexaFluor-568
(Invitrogen, A11004)
DAPI (Sigma, D9542) DAPI (Unkown) DAPI (Sigma, D9542) Hoechst (Invitrogen, H3570)
Palloidin AlexaFluor-555 Phalloidin (Invitrogen, A34055)
Project Release Updates
KGP 1.1.1 – Released May 27, 2021 – Add 106 Split-Gal4 Data and Images
KGP 1.1.0 – Released December 23, 2020 – Add LS images
KGP 1.0.0 – Released October 28, 2020
Total Lines: 612, Total Images: 2090
For error reporting
Dr. Sung-Eun Yoon (KDRC, kdrc@gist.ac.kr)

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