한국초파리연구자원은행 KDRC > K-Gut Project

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Split Gal4 Images
K-GUT ID JK R61H08 X R27E07
DBD
BDSC ID 69158 FlyLight ID R61H08 Associated Gene AstC
AD
BDSC ID 69855 FlyLight ID R27E07 Associated Gene l(2)gl
Crop X PV X R1 X
R2 X R3 X R4 X
R5 X MHJ X Hindgut X
Rectum X

Split-Gal4 associated image

Associated Split Gal4 Images
K-GUT ID DBD AD
BDSC ID FlyLight ID Associated Gene BDSC ID FlyLight ID Associated Gene
JK R61H08 X R65D05 69158 R61H08 AstC 69989 R65D05 AstA
JK R61H08 X R67F03 69158 R61H08 AstC 70786 R67F03 AstC
JK R61H08 X R17D02 69158 R61H08 AstC 69078 R17D02 fkh
JK R61H08 X R46D01 69158 R61H08 AstC 70700 R46D01 hth
JK R61H08 X R60H02 69158 R61H08 AstC 68901 R60H02 MsR1
JK R61H08 X R16E08 69158 R61H08 AstC 68946 R16E08 noc
JK R61H08 X R69H11 69158 R61H08 AstC 71120 R69H11 vnd
JK R57F07 X R27E07 70283 R57F07 Dh31 69855 R27E07 l(2)gl
JK R61H08 X R57F07 69158 R61H08 AstC 71151 R57F07 Dh31
JK R27E07 X R57F07 69810 R27E07 l(2)gl 71151 R57F07 Dh31

Gal4 associated image

Associated Gal4 Images
K-GUT ID Gal4
BDSC ID FlyLight ID Associated Gene
GS39283_5 39283 R61H08 AstC
WL49225_4 49225 R27E07 l(2)gl

K-GUT Project

K-GUT project is an on-going project of KDRC, which aims to archive Drosophila gut GAL4 transgenes and their expression patterns. K-GUT project provides a database accessible to all researchers in the Drosophila community.

Principal Investigators Participating in K-GUT Project

Greg S. B. Suh
KAIST, seongbaesuh@kaist.ac.kr
Jae Young Kwon
Sungkyunkwan University, jykwon@skku.edu
Won-Jae Lee
Seoul National University, lwj@snu.ac.kr
Young-Joon Kim
GIST, kimyj@gist.ac.kr

Publication

Seung Yeon Lim, Hyejin You, Jinhyeong Lee, Jaejin Lee, Yoojin Lee, Kyung-Ah Lee, Boram Kim, Ji-Hoon Lee, JiHyeon Jeong, Sooin Jang, Byoungsoo Kim, Hyungjun Choi, Gayoung Hwang, Min Sung Choi, Sung-Eun Yoon, Jae Young Kwon, Won-Jae Lee, Young-Joon Kim & Greg S. B. Suh (2020) Identification and characterization of GAL4 drivers that mark distinct cell types and regions in the Drosophila adult gut, Journal of Neurogenetics, DOI: 10.1080/01677063.2020.1853722
For reprint requests, kdrc@gist.ac.kr

Imaging Conditions

K-GUT ID GS JK WL LS
Microscopy Zeiss LSM 780 Zeiss LSM 700 Zeiss LSM 700 Zeiss Lightsheet Z.1
Lens 20X
(Plan-Apochromat 20x/0.8 M27)
20X 20X
(Plan Apochromat 20x/0.8)
10X
(W Plan-Apochromat 10x/0.5)
Antibodies GFP (Invitrogen, A11122) GFP (Invitrogen, A11122) GFP (Invitrogen, A11122) GFP (Invitrogen, A6455)
Prospero (DSHB, MR1A) Prospero (DSHB, MR1A) Prospero (DSHB, MR1A)
AlexaFluor-488
(Invitrogen, A21206)
AlexaFluor-488
(Invitrogen, A11034)
AlexaFluor-488
(Invitrogen, A11008)
AlexaFluor-488
(Invitrogen, A32723)
AlexaFluor-594
(Invitrogen, A21203)
AlexaFluor-568
(Invitrogen, A11031)
AlexaFluor-568
(Invitrogen, A11004)
DAPI (Sigma, D9542) DAPI (Unkown) DAPI (Sigma, D9542) Hoechst (Invitrogen, H3570)
Palloidin AlexaFluor-555 Phalloidin (Invitrogen, A34055)
Project Release Updates
KGP 1.1.2 – Released May 18, 2022 – Add 360 Gal4 Data and 1,121 Images
KGP 1.1.1 – Released May 27, 2021 – Add 105 Split-Gal4 Data and Images
KGP 1.1.0 – Released December 23, 2020 – Add 199 Light-sheet images
KGP 1.0.0 – Released October 28, 2020
Total Lines: 973, Total Images: 3,222
For error reporting
Dr. Sung-Eun Yoon (KDRC, kdrc@gist.ac.kr)

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