한국초파리연구자원은행 KDRC > K-Gut Project

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Split Gal4 Images
K-GUT ID GS R41A11 X R33B09
DBD
BDSC ID 70381 FlyLight ID R41A11 Associated Gene psq
AD
BDSC ID 75724 FlyLight ID R33B09 Associated Gene SNF4Agamma
Crop O PV O R1 O
R2 X R3 X R4 X
R5 O MHJ O Hindgut O
Rectum O

Split-Gal4 associated image

Associated Split Gal4 Images
K-GUT ID DBD AD
BDSC ID FlyLight ID Associated Gene BDSC ID FlyLight ID Associated Gene
GS R35F11 X R33B09 68671 R35F11 CG32180 75724 R33B09 SNF4Agamma
GS R10B09 X R33B09 69900 R10B09 Fas2 75724 R33B09 SNF4Agamma
GS R33B09 X R33B09 68760 R33B09 SNF4Agamma 75724 R33B09 SNF4Agamma
GS R33B09 X R75G05 68760 R33B09 SNF4Agamma 70952 R75G05 CG9674
GS R33B09 X R35F11 68760 R33B09 SNF4Agamma 68713 R35F11 CG32180
GS R33B09 X R84D10 68760 R33B09 SNF4Agamma 70834 R84D10 Eip93F
GS R33B09 X R17D02 68760 R33B09 SNF4Agamma 69078 R17D02 fkh
GS R33B09 X R22E09 68760 R33B09 SNF4Agamma 71003 R22E09 fru
GS R33B09 X R10F07 68760 R33B09 SNF4Agamma 68811 R10F07 Fas2
GS R41A11 X R75G05 70381 R41A11 psq 70952 R75G05 CG9674
GS R41A11 X R35F11 70381 R41A11 psq 68713 R35F11 CG32180
GS R41A11 X R17D02 70381 R41A11 psq 69078 R17D02 fkh
GS R41A11 X R22E09 70381 R41A11 psq 71003 R22E09 fru
GS R41A11 X R10F07 70381 R41A11 psq 68811 R10F07 Fas2
GS R41A11 X R56E09 70381 R41A11 psq 68886 R56E09 foxo
GS R84D10 X R33B09 70451 R84D10 Eip93F 75724 R33B09 SNF4Agamma
GS R17D02 X R33B09 69173 R17D02 fkh 75724 R33B09 SNF4Agamma
GS R10F07 X R33B09 69005 R10F07 Fas2 75724 R33B09 SNF4Agamma
GS R22E09 X R33B09 68963 R22E09 fru 75724 R33B09 SNF4Agamma
GS R56E09 X R33B09 70296 R56E09 foxo 75724 R33B09 SNF4Agamma
GS R75G05 X R33B09 71153 R75G05 CG9674 75724 R33B09 SNF4Agamma

Gal4 associated image

Associated Gal4 Images
K-GUT ID Gal4
BDSC ID FlyLight ID Associated Gene
JK50109_10 50109 R41A11 psq
JK49361_10 49361 R33B09 SNF4Agamma

K-GUT Project

K-GUT project is an on-going project of KDRC, which aims to archive Drosophila gut GAL4 transgenes and their expression patterns. K-GUT project provides a database accessible to all researchers in the Drosophila community.

Principal Investigators Participating in K-GUT Project

Greg S. B. Suh
KAIST, seongbaesuh@kaist.ac.kr
Jae Young Kwon
Sungkyunkwan University, jykwon@skku.edu
Won-Jae Lee
Seoul National University, lwj@snu.ac.kr
Young-Joon Kim
GIST, kimyj@gist.ac.kr

Publication

Seung Yeon Lim, Hyejin You, Jinhyeong Lee, Jaejin Lee, Yoojin Lee, Kyung-Ah Lee, Boram Kim, Ji-Hoon Lee, JiHyeon Jeong, Sooin Jang, Byoungsoo Kim, Hyungjun Choi, Gayoung Hwang, Min Sung Choi, Sung-Eun Yoon, Jae Young Kwon, Won-Jae Lee, Young-Joon Kim & Greg S. B. Suh (2020) Identification and characterization of GAL4 drivers that mark distinct cell types and regions in the Drosophila adult gut, Journal of Neurogenetics, DOI: 10.1080/01677063.2020.1853722
For reprint requests, kdrc@gist.ac.kr

Imaging Conditions

K-GUT ID GS JK WL LS
Microscopy Zeiss LSM 780 Zeiss LSM 700 Zeiss LSM 700 Zeiss Lightsheet Z.1
Lens 20X
(Plan-Apochromat 20x/0.8 M27)
20X 20X
(Plan Apochromat 20x/0.8)
10X
(W Plan-Apochromat 10x/0.5)
Antibodies GFP (Invitrogen, A11122) GFP (Invitrogen, A11122) GFP (Invitrogen, A11122) GFP (Invitrogen, A6455)
Prospero (DSHB, MR1A) Prospero (DSHB, MR1A) Prospero (DSHB, MR1A)
AlexaFluor-488
(Invitrogen, A21206)
AlexaFluor-488
(Invitrogen, A11034)
AlexaFluor-488
(Invitrogen, A11008)
AlexaFluor-488
(Invitrogen, A32723)
AlexaFluor-594
(Invitrogen, A21203)
AlexaFluor-568
(Invitrogen, A11031)
AlexaFluor-568
(Invitrogen, A11004)
DAPI (Sigma, D9542) DAPI (Unkown) DAPI (Sigma, D9542) Hoechst (Invitrogen, H3570)
Palloidin AlexaFluor-555 Phalloidin (Invitrogen, A34055)
Project Release Updates
KGP 1.1.1 – Released May 27, 2021 – Add 106 Split-Gal4 Data and Images
KGP 1.1.0 – Released December 23, 2020 – Add LS images
KGP 1.0.0 – Released October 28, 2020
Total Lines: 612, Total Images: 2090
For error reporting
Dr. Sung-Eun Yoon (KDRC, kdrc@gist.ac.kr)

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